José Fernando Garcia
Universidade Estadual Paulista Júlio de Mesquita Filho, Brazil
Graduated from the Faculty of Veterinary Medicine, University of Sao Paulo (1989), Master of Veterinary Science, Federal University of Rio Grande do Sul (1992) and Ph.D. in Animal Reproduction at the University of São Paulo (1995). He is currently Associate Professor at Universidade Estadual Paulista Julio de Mesquita Filho, having previously been professor at the University of São Paulo (1995-1997) and scientist at the Joint FAO / IAEA Programme Nuclear Techniques in Food and Agriculture (2003 to 2005). Coordinates the Laboratory of Animal Biochemistry and Molecular Biology, UNESP - Araçatuba, part of the Collaborating Centre for the International Atomic Energy Agency (IAEA) in Animal Genomics and Bioinformatics. Acting in different fields of Animal Biotechnology, in particular the following issues: selection and breeding assisted by molecular markers in livestock and diagnosis of infectious and parasitic diseases in animals by the use of related methods for nucleic acid analysis and genomic approaches. Also involved in international consortiums for genome sequencing and analytical tools development such as: Bos taurus, Bos indicus, Bus bubalis (water buffalo) and Capra hircus (goat). Active member of the International Society of Animal Genetics (ISAG). In Brazil, was Treasurer of the Brazilian Society of Embryo Technology (SBTE) for two terms (96/97 and 98/99) and its President (2008/2010). In 2010 completed an MBA at Fundacao Getulio Vargas in Sao Paulo, in the area of Strategic Management and Economic Affairs. Member of the National Biosafety Technical Commission (CTNBio) of the Ministry of Science and Technology during the biennium 2010 to 2011.
From the assembly of a "de novo" genome to its functional exploration: The short story of Bos indicus.
In order to explore the vast possibilities offered by genomics, the starting point is the existence of a reference assembly, which one can trust in order to build new hypothesis and make correct assumptions. In the case of mammals, except for human, some primates and laboratory animals, there are still few examples of high quality reference genomes. The bovine was the first livestock species to have it accomplished (first draft of Bos taurus released in 2009), even though it is still being updated. In 2009, a new initiative was started in order to produce a "de novo" assembly of the bovine sub-species Bos indicus, the most important for tropical regions of the world (particularly to Brazil). The first draft of this assembly is ready and new windows for exploration are being opened right now. Exome capture analysis, RNAseq, SNP chip development, genome wide association studies and genomic selection are just some of the possible approaches to help us to develop new tools for better understanding and exploring livestock as the protein producing machines they represent. This talk will present and discuss these approaches in practical perspectives.